Tagged-Cap
(Plasmid #35572)

• Purpose: (Empty Backbone)
• Depositing Lab: Marian Waterman
• Publication: Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110.007385. Epub 2011 Feb 1.
• Price: $65 (USD)

Backbone

• Vector backbone: pRSTF
  (Search Vector Database)
• Backbone manufacturer: Jang et al. 2004
• Backbone size (bp): 4660
• Modifications to backbone: Renilla Luciferase open reading frame removed. 4 Ms2 stem loop sequences cloned downstream of Firefly Luciferase.
• Vector type: Mammalian Expression, Luciferase
• Promoter: SV40
• Tag / Fusion Protein:
  • Ms2 Stem Loop (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Sequence Information

• Depositor Sequences:
  Full (1)
• Addgene Sequences:
  Partial (1)

Resource Information

• Supplemental documents:
  • 35572 sequence
• Terms and Licenses:
  • UBMTA
  • Luciferase Limited Use Label License