Addgene - Tagged-Cap Plasmid Data

Plasmid 35572: Tagged-Cap

Gene/insert name:
None
Vector backbone:
pRSTF
(Search Vector Database)
Backbone manufacturer:
Jang et al. 2004
Vector type:
Mammalian Expression, Luciferase
Backbone size (bp):
4660
Modifications to Backbone:
Renilla Luciferase open reading frame removed. 4 Ms2 stem loop sequences cloned downstream of Firefly Luciferase.
Promoter:
SV40
Fusion protein or tag:
Ms2 Stem Loop
Terminal:
C terminal on backbone
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
35572 sequence (image/jpeg)
Principal Investigator:
Marian Waterman
Terms and Licenses:
UBMTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Quantitative profiling of in vivo-assembled RNA-protein complexes using a novel integrated proteomic approach. Tsai et al (Mol Cell Proteomics. 2011 Apr;10(4):M110.007385. Epub 2011 Feb 1. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35572" in your Materials and Methods section.