Plasmid 35572: Tagged-Cap

• Gene/insert name: None
• Vector backbone: pRSTF
  (Search Vector Database)
• Backbone manufacturer: Jang et al. 2004
• Vector type: Mammalian Expression, Luciferase
• Backbone size (bp): 4660
• Modifications to Backbone: Renilla Luciferase open reading frame removed. 4 Ms2 stem loop sequences cloned downstream of Firefly Luciferase.
• Promoter: SV40
• Fusion protein or tag: Ms2 Stem Loop
• Terminal: C terminal on backbone
• Bacterial resistance(s) Ampicillin
• Growth strain(s) DH5alpha
• Growth temperature (℃): 37
• High or low copy: High Copy
• Sequence: View sequences (2)
• Map: View map
• Supplemental document: 35572 sequence (image/jpeg)
• Principal Investigator: Marian Waterman
• Terms and Licenses: MTA
  Luciferase Limited Use Label License

Article: Quantitative profiling of in vivo-assembled RNA-protein complexes using a novel integrated proteomic approach. Tsai et al (Mol Cell Proteomics. 2011 Apr;10(4):M110.007385. Epub 2011 Feb 1. PubMed)