Tagged-Cap
(Plasmid #35572)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pRSTF
  • Backbone manufacturer
    Jang et al. 2004
  • Backbone size (bp) 4660
  • Modifications to backbone
    Renilla Luciferase open reading frame removed. 4 Ms2 stem loop sequences cloned downstream of Firefly Luciferase.
  • Vector type
    Mammalian Expression, Luciferase
  • Promoter SV40
  • Tag / Fusion Protein
    • Ms2 Stem Loop (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tagged-Cap was a gift from Marian Waterman (Addgene plasmid # 35572)
  • For your References section:

    Quantitative profiling of in vivo-assembled RNA-protein complexes using a novel integrated proteomic approach. Tsai BP, Wang X, Huang L, Waterman ML. Mol Cell Proteomics. 2011 Apr;10(4):M110.007385. Epub 2011 Feb 1. 10.1074/mcp.M110.007385 PubMed 21285413