pBPHLWL
(Plasmid #26254)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBluescript II KS(-)
  • Backbone size w/o insert (bp) 7929
  • Vector type
    Bacterial Expression, Insect Expression ; Drosophila transgenesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    Multiple cloning site
  • Insert Size (bp)
    34

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBPHLWL was a gift from Tom Clandinin (Addgene plasmid # 26254)
  • For your References section:

    A versatile in vivo system for directed dissection of gene expression patterns. Gohl D, Silies M, Gao X, Bhalerao S, Luongo F, Lin CC, Potter C, Clandinin T. Nature Methods (2011) doi:10.1038/nmeth.1561 10.1038/nmeth.1561