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pTomo
(Plasmid #26291)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    p156RRLsin PPTCMVIRESPRE
  • Backbone manufacturer
    Naldini lab
  • Backbone size (bp) 8753
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    empty

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

To generate pTomo, the inserted loxP-mRFP-loxP fragment amplified from the pSETB mRFP1 vector by PCR between the XbaI and BamHI sites of the p156RRLsin PPTCMVIRESPRE vector (a third-generation lentiviral vector).

Note that the Addgene quality control sequence detected a G->A mutation in the IRES. It is not known if or how this mutation affects function.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTomo was a gift from Inder Verma (Addgene plasmid # 26291)
  • For your References section:

    Development of a novel mouse glioma model using lentiviral vectors. Marumoto T, Tashiro A, Friedmann-Morvinski D, Scadeng M, Soda Y, Gage FH, Verma IM. Nat Med. 2009 Jan . 15(1):110-6. 10.1038/nm.1863 PubMed 19122659