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Plasmid 26291: pTomo
  • empty

  • p156RRLsin PPTCMVIRESPRE
    (Search Vector Database)

  • Naldini lab

  • Lentiviral

  • 8753

  • XbaI

  • No

  • BamHI

  • No

  • CMV-F List of Sequencing Primers

  • IRES-R

  • Ampicillin

  • Stbl3

  • 37

  • Low Copy

  • View sequences (2)
  • Inder Verma

  • UBMTA

Comments: 

To generate pTomo, the inserted loxP-mRFP-loxP fragment amplified from the pSETB mRFP1 vector by PCR between the XbaI and BamHI sites of the p156RRLsin PPTCMVIRESPRE vector (a third-generation lentiviral vector).

Note that the Addgene quality control sequence detected a G->A mutation in the IRES. It is not known if or how this mutation affects function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Development of a novel mouse glioma model using lentiviral vectors. Marumoto et al (Nat Med. 2009 Jan . 15(1):110-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26291" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only