Addgene - pTomo Plasmid Data

Plasmid 26291: pTomo

Gene/insert name:
empty
Vector backbone:
p156RRLsin PPTCMVIRESPRE
(Search Vector Database)
Backbone manufacturer:
Naldini lab
Vector type:
Lentiviral
Backbone size (bp):
8753
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
IRES-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (2)
Principal Investigator:
Inder Verma
Terms and Licenses:
MTA

Comments:

To generate pTomo, the inserted loxP-mRFP-loxP fragment amplified from the pSETB mRFP1 vector by PCR between the XbaI and BamHI sites of the p156RRLsin PPTCMVIRESPRE vector (a third-generation lentiviral vector).

Note that the Addgene quality control sequence detected a G->A mutation in the IRES. It is not known if or how this mutation affects function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Development of a novel mouse glioma model using lentiviral vectors. Marumoto et al (Nat Med. 2009 Jan . 15(1):110-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26291" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

18723: CMV-Brainbow-2.1 R