Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26306||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7711
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Alt namemir 124
SpeciesH. sapiens (human)
Insert Size (bp)443
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer V5 reverse (5â€²-ACCGAGGAGAGGGTTAGGGAT-3â€² ) (Common Sequencing Primers)
pre-miRNA hairpins and the
surrounding regions were amplified from human genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.2/V5 hsa-mir-124 was a gift from David Bartel (Addgene plasmid # 26306 ; http://n2t.net/addgene:26306 ; RRID:Addgene_26306)
For your References section:Mammalian microRNAs: experimental evaluation of novel and previously annotated genes. Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP. Genes Dev. 2010 May 15. 24(10):992-1009. 10.1101/gad.1884710 PubMed 20413612