1
Browse > Bob Weinberg > Ma et al > pBabe-puro-miR-9 sponge
Plasmid 25040: pBabe-puro-miR-9 sponge

  • miR-9 sponge

  • 186

  • pBabe-puro
    (Search Vector Database)

  • Bob, Weinberg, Addgene plasmid 1764

  • Mammalian Expression, Retroviral

  • 5169

  • XhoI

  • Unknown

  • ApaI

  • Unknown

  • pBABE 5' List of Sequencing Primers

  • pBABE 3'

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Puromycin

  • View sequences (2)

  • Bob Weinberg

  • MTA

Comments: 

8 tandem binding sites to human miR-9. Repeated sequence is TCATACAGCTATATACCAAAGA.

Annealed oligonucleotides for tandem miR-9-binding sites were ligated into the pcDNA5-CMV-d2eGFP vector (Invitrogen) digested with XhoI and ApaI. The gfp mRNA, along with the miR-9 sponge sequence in the 3′ UTR was then subcloned into the pBabe-puro vector digested with BamHI and SalI.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: miR-9, a MYC/MYCN-activated microRNA, regulates E-cadherin and cancer metastasis. Ma et al (Nat Cell Biol. 2010 Mar . 12(3):247-56. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25040" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only