Addgene - MDH1-PGK-GFP-miR9 Plasmid Data

Plasmid 25036: MDH1-PGK-GFP-miR9

Gene/insert name:
mir-9-3
Insert size:
313
Species:
H. sapiens (human)
Entrez Gene:
MIR9-3 (MIRN9-3, miRNA9-3, hsa-mir-9-3)
Vector backbone:
MDH1-PGK-GFP 2.0
(Search Vector Database)
Backbone manufacturer:
Chang-Zheng Chen, Addgene plasmid 11375
Vector type:
Mammalian Expression, Retroviral, RNAi
Backbone size w/o insert (bp):
6963
Cloning site 5':
EcoRI
Site destroyed during cloning:
Unknown
Cloning site 3':
XhoI
Site destroyed during cloning:
Unknown
5' sequencing primer:
EGFP-C List of Sequencing Primers
3' sequencing primer:
H1, tcgctatgtgttctgggaaa
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

The mir-9-3 gene was PCR-amplified from normal human genomic DNA and cloned into the MDH-PGK-GFP 2.0 retroviral vector. It includes the miR-9 stem-loop and ~100bp flanking sequences on both sides.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: miR-9, a MYC/MYCN-activated microRNA, regulates E-cadherin and cancer metastasis. Ma et al (Nat Cell Biol. 2010 Mar . 12(3):247-56. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25036" in your Materials and Methods section.