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Plasmid 25037: pMIR-9
  • miR-9 binding site

  • microRNA 9

  • 36

  • H. sapiens (human)

  • pMIR-report
    (Search Vector Database)

  • Ambion

  • Mammalian Expression, Luciferase

  • 6470

  • SpeI

  • Unknown

  • HindIII

  • Unknown

  • Luc-F List of Sequencing Primers

  • M13/pUC Forward

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Puromycin

  • View sequences (1)
  • Bob Weinberg

  • UBMTA
    Luciferase Limited Use Label License

Comments: 

Human miR-9 binding site (the reverse complementary sequence of miR-9) was generated by annealing of 2 oligos (5′-CTAGTTCATACAGCTAGATAACCAAAGAGCTCAGCA-3′ (forward) and 5′-AGCTTGCTGAGCTCTTTGGTTATCTAGCTGTATGAA-3′ (reverse)), and ligating into pMIR-report.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: miR-9, a MYC/MYCN-activated microRNA, regulates E-cadherin and cancer metastasis. Ma et al (Nat Cell Biol. 2010 Mar . 12(3):247-56. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25037" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only