Addgene - pMIR-9 Plasmid Data

Plasmid 25037: pMIR-9

Gene/insert name:
miR-9 binding site
Alt name:
microRNA 9
Insert size:
36
Species:
H. sapiens (human)
Vector backbone:
pMIR-report
(Search Vector Database)
Backbone manufacturer:
Ambion
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
6470
Cloning site 5':
SpeI
Site destroyed during cloning:
Unknown
Cloning site 3':
HindIII
Site destroyed during cloning:
Unknown
5' sequencing primer:
Luc-F List of Sequencing Primers
3' sequencing primer:
M13/pUC Forward
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

Human miR-9 binding site (the reverse complementary sequence of miR-9) was generated by annealing of 2 oligos (5′-CTAGTTCATACAGCTAGATAACCAAAGAGCTCAGCA-3′ (forward) and 5′-AGCTTGCTGAGCTCTTTGGTTATCTAGCTGTATGAA-3′ (reverse)), and ligating into pMIR-report.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: miR-9, a MYC/MYCN-activated microRNA, regulates E-cadherin and cancer metastasis. Ma et al (Nat Cell Biol. 2010 Mar . 12(3):247-56. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25037" in your Materials and Methods section.