|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25037||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6470
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemiR-9 binding site
Alt namemicroRNA 9
SpeciesH. sapiens (human)
Insert Size (bp)36
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer Luc-F
- 3′ sequencing primer M13/pUC Forward (Common Sequencing Primers)
Human miR-9 binding site (the reverse complementary sequence of miR-9) was generated by annealing of 2 oligos (5′-CTAGTTCATACAGCTAGATAACCAAAGAGCTCAGCA-3′ (forward) and 5′-AGCTTGCTGAGCTCTTTGGTTATCTAGCTGTATGAA-3′ (reverse)), and ligating into pMIR-report.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMIR-9 was a gift from Bob Weinberg (Addgene plasmid # 25037)
For your References section:miR-9, a MYC/MYCN-activated microRNA, regulates E-cadherin and cancer metastasis. Ma L, Young J, Prabhala H, Pan E, Mestdagh P, Muth D, Teruya-Feldstein J, Reinhardt F, Onder TT, Valastyan S, Westermann F, Speleman F, Vandesompele J, Weinberg RA. Nat Cell Biol. 2010 Mar . 12(3):247-56. 10.1038/ncb2024 PubMed 20173740