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pMIR-CDH1 mutUTR
(Plasmid #25039)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25039 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMIR-report
  • Backbone manufacturer
    Ambion
  • Backbone size w/o insert (bp) 6470
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CDH1 mutant 3'UTR
  • Alt name
    CDH1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    819
  • Mutation
    substitution of 6nt within the miR-9 binding site from CAAAGA to GTTTCT.
  • Entrez Gene
    CDH1 (a.k.a. Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer Luc-F
  • 3′ sequencing primer M13/pUC Forward
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

A partial CDH1 3'UTR (819bp) was PCR-amplified from normal human genomic DNA and cloned into the pMIR-report vector. The mutant construct of the CDH1 3′ UTR was generated with a QuikChange Site-Directed Mutagenesis Kit (Stratagene).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIR-CDH1 mutUTR was a gift from Bob Weinberg (Addgene plasmid # 25039 ; http://n2t.net/addgene:25039 ; RRID:Addgene_25039)
  • For your References section:

    miR-9, a MYC/MYCN-activated microRNA, regulates E-cadherin and cancer metastasis. Ma L, Young J, Prabhala H, Pan E, Mestdagh P, Muth D, Teruya-Feldstein J, Reinhardt F, Onder TT, Valastyan S, Westermann F, Speleman F, Vandesompele J, Weinberg RA. Nat Cell Biol. 2010 Mar . 12(3):247-56. 10.1038/ncb2024 PubMed 20173740