Addgene - pFRT_DESTFLAGHA Plasmid Data

Plasmid 26360: pFRT_DESTFLAGHA

Gene/insert name:
None
Fusion protein or tag:
FLAG/HA
Terminal:
N terminal on backbone
Vector backbone:
pcDNA5/FRT/TO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size (bp):
5137
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGH-Rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
ccdB Survival
Growth temperature (℃):
37
Growth instructions:
ccdB survival
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Thomas Tuschl
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: mRNA and protein levels of FUS, EWSR1, and TAF15 are upregulated in liposarcoma. Spitzer et al (Genes Chromosomes Cancer. 2011 May . 50(5):338-47. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26360" in your Materials and Methods section.