Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26360||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5137
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsccdB survival
Copy numberHigh Copy
/ Fusion Protein
- FLAG/HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFRT_DESTFLAGHA was a gift from Thomas Tuschl (Addgene plasmid # 26360 ; http://n2t.net/addgene:26360 ; RRID:Addgene_26360)
For your References section:mRNA and protein levels of FUS, EWSR1, and TAF15 are upregulated in liposarcoma. Spitzer JI, Ugras S, Runge S, Decarolis P, Antonescu C, Tuschl T, Singer S. Genes Chromosomes Cancer. 2011 May . 50(5):338-47. 10.1002/gcc.20858 PubMed 21344536