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pFRT_DESTFLAGHA
(Plasmid #26360)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26360 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA5/FRT
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 5137
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    ccdB survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • FLAG/HA (N terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT_DESTFLAGHA was a gift from Thomas Tuschl (Addgene plasmid # 26360 ; http://n2t.net/addgene:26360 ; RRID:Addgene_26360)
  • For your References section:

    mRNA and protein levels of FUS, EWSR1, and TAF15 are upregulated in liposarcoma. Spitzer JI, Ugras S, Runge S, Decarolis P, Antonescu C, Tuschl T, Singer S. Genes Chromosomes Cancer. 2011 May . 50(5):338-47. 10.1002/gcc.20858 PubMed 21344536