|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26422||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3380
Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameHSV-TK, CS2-Venus
Insert Size (bp)5180
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI, SalI, Sse8387I (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBRHDAd-TKVenus was a gift from Kohnosuke Mitani (Addgene plasmid # 26422 ; http://n2t.net/addgene:26422 ; RRID:Addgene_26422)
For your References section:Highly efficient transient gene expression and gene targeting in primate embryonic stem cells with helper-dependent adenoviral vectors. Suzuki K, Mitsui K, Aizawa E, Hasegawa K, Kawase E, Yamagishi T, Shimizu Y, Suemori H, Nakatsuji N, Mitani K. Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13781-6. 10.1073/pnas.0806976105 PubMed 18768795