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pAMHDAdGT8-4
(Plasmid #26421)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26421 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBR322
  • Backbone size w/o insert (bp) 3360
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HSV-TK, mCMV-b-gal
  • Species
    E.coli, HSV
  • Insert Size (bp)
    8240

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI, SpeI, NheI, SalI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

There are some discrepancies between Addgene's quality control sequence and the author's sequence, but the Mitani lab has confirmed that this plasmid is correct. The depositor has also confirmed that there is an extra SacI site present in the plasmid is not apparent in the theoretical sequence on which the map below is based. Addgene does not recommend using SacI for a diagnostic digest.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAMHDAdGT8-4 was a gift from Kohnosuke Mitani (Addgene plasmid # 26421 ; http://n2t.net/addgene:26421 ; RRID:Addgene_26421)