|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26421||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3360
Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameHSV-TK, mCMV-b-gal
Insert Size (bp)8240
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI, SpeI, NheI, SalI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
There are some discrepancies between Addgene's quality control sequence and the author's sequence, but the Mitani lab has confirmed that this plasmid is correct. The depositor has also confirmed that there is an extra SacI site present in the plasmid is not apparent in the theoretical sequence on which the map below is based. Addgene does not recommend using SacI for a diagnostic digest.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAMHDAdGT8-4 was a gift from Kohnosuke Mitani (Addgene plasmid # 26421 ; http://n2t.net/addgene:26421 ; RRID:Addgene_26421)