Addgene - p172 Dync1i2.E (Ex 1b) Plasmid Data

Plasmid 26442: p172 Dync1i2.E (Ex 1b)

Gene/insert name:
cytoplasmic dynein 1 intermediate chain 2 isoform E (exon 1b)
Alt name:
Dync1i2, IC74, DIC
Insert size:
2516
Species:
M. musculus (mouse)
GenBank ID:
NM_010064
Mutation:
N/A
Vector backbone:
pCR4-TOPO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
subcloning and sequencing
Backbone size w/o insert (bp):
3956
Cloning method:
TOPO Cloning
5' sequencing primer:
M13, T7 List of Sequencing Primers
3' sequencing primer:
M13, T7
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10 [Invitrogen], standard LB with 50 ug/ml of kanamicin
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Elizabeth Fisher
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mouse cytoplasmic dynein intermediate chains: identification of new isoforms, alternative splicing and tissue distribution of transcripts. Kuta et al (PLoS One. 2010 . 5(7):e11682. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26442" in your Materials and Methods section.