Addgene - PP1 7-300 Plasmid Data

Plasmid 26566: PP1 7-300

Gene/insert name:
PP1a
Alt name:
PP1 alpha
Insert size:
879
Species:
H. sapiens (human)
Entrez Gene:
PPP1CA (PP-1A, PP1A, PP1alpha, PPP1A)
Fusion protein or tag:
Thio-6
Terminal:
N terminal on backbone
Fusion protein or tag:
His6
Terminal:
N terminal on backbone
Fusion protein or tag:
TEV cleavage site
Terminal:
N terminal on backbone
Mutation:
Contains only aa 7-330
Vector backbone:
RP1B
(Search Vector Database)
Backbone manufacturer:
Peti Lab (Addgene #26563)
Vector type:
Bacterial Expression
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T7-term
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Wolfgang Peti
Terms and Licenses:
MTA

Comments:

The backbone is a modified version of pET28 and is also available at Addgene (plasmid #26563)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Crystal structures of protein phosphatase-1 bound to nodularin-R and tautomycin: a novel scaffold for structure-based drug design of serine/threonine phosphatase inhibitors. Kelker et al (J Mol Biol. 2009 Jan 9. 385(1):11-21. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26566" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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