Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26613||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerShirley Tilghman lab
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameIgf2 enhancer
SpeciesM. musculus (mouse)
Insert Size (bp)4000
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (created at XhoI) (not destroyed)
- 3′ cloning site EagI (created at XhoI) (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS-H19-luciferase ME was a gift from Jie Chen (Addgene plasmid # 26613 ; http://n2t.net/addgene:26613 ; RRID:Addgene_26613)
For your References section:IGF-II transcription in skeletal myogenesis is controlled by mTOR and nutrients. Erbay E, Park IH, Nuzzi PD, Schoenherr CJ, Chen J. J Cell Biol. 2003 Dec 8. 163(5):931-6. 10.1083/jcb.200307158 PubMed 14662739