Addgene - pLKO.1 RagB shRNA #2 Plasmid Data

Plasmid 26628: pLKO.1 RagB shRNA #2

Gene/insert name:
RagB shRNA
Alt name:
RagB
Species:
H. sapiens (human)
Entrez Gene:
RRAGB (RP11-465E19.1, RAGB, bA465E19.1)
Vector backbone:
pLKO.1 puro
(Search Vector Database)
Backbone manufacturer:
Available at Addgene (#8453)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert (bp):
7000
Cloning site 5':
AgeI
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoRI
Site destroyed during cloning:
Unknown
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
David Sabatini
Terms and Licenses:
MTA

Comments:

shRNA directed against RagB. Target sequence: 5'-GCCTCTTTCCAGAGTATGGAA-3'. Sequence from the website of the RNAi consortium at the Broad institute.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al (Science. 2008 Jun 13. 320(5882):1496-501. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26628" in your Materials and Methods section.