PCDNA-Δ90AEGFP
(Plasmid
#26644)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26644 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameΔ90beta catenin
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SpeciesM. musculus (mouse)
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Insert Size (bp)2907
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MutationDeletion of first 90 aa of beta catenin
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Tag
/ Fusion Protein
- EGFP-mut4 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byEGFP-mut4 is from Ami Okada
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Δ90AEGFP is Δ90betacatenin fused with EGFP-mut4 from Ami Okada
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PCDNA-Δ90AEGFP was a gift from Anjen Chenn (Addgene plasmid # 26644 ; http://n2t.net/addgene:26644 ; RRID:Addgene_26644)