pRL-TK 3'FBXW7 UTR
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26649||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4045
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1571
MutationContains only the 3'UTR of FBXW7, nucleotides 1-1571 following stop codon.
Entrez GeneFBXW7 (a.k.a. AGO, CDC4, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4)
/ Fusion Protein
- Luciferase (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer EBV-Rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Please note that Addgene's sequencing results identified nucleotide mismatches when compared to GenBank reference sequence NM_018315.4 at basepair #'s 2389, 2663, 2666, 2829 and 3526.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRL-TK 3'FBXW7 UTR was a gift from Alex Minella (Addgene plasmid # 26649 ; http://n2t.net/addgene:26649 ; RRID:Addgene_26649)
For your References section:MicroRNA-223 regulates cyclin E activity by modulating expression of F-box and WD-40 domain protein 7. Xu Y, Sengupta T, Kukreja L, Minella AC. J Biol Chem. 2010 Sep 7. ():. 10.1074/jbc.M110.152306 PubMed 20826802