Addgene - RFP-zyxin Plasmid Data

Plasmid 26720: RFP-zyxin

Gene/insert name:
Zyxin
Species:
H. sapiens (human)
Species:
unknown
Fusion protein or tag:
RFP
Terminal:
C terminal on backbone
Vector backbone:
pDsRed-N1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression, Bacterial Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Anna Huttenlocher
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Regulation of focal complex composition and disassembly by the calcium-dependent protease calpain. Bhatt et al (J Cell Sci. 2002 Sep 1. 115(Pt 17):3415-25. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26720" in your Materials and Methods section.