Addgene - pEGFP-N1 alpha-actinin 1 Plasmid Data

Gene/insert name:
alpha-actinin 1
Alt name:
ACTN1
Insert size:
2685
Species:
H. sapiens (human)
GenBank ID:
NM_001102
Entrez Gene:
ACTN1 (FLJ40884, FLJ54432)
Fusion protein or tag:
eGFP
Terminal:
C terminal on backbone
Vector backbone:
pEGFP-N1
(Search Vector Database)
Backbone manufacturer:
Clontech Laboratories
Vector type:
Mammalian Expression
Backbone size w/o insert:
4700
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
EGFP-N Sequencing Primer
Bacterial resistance:
Kanamycin
Growth strain:
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Dr. D.J. Kwiatkowski (Harvard Medical School) provided non-muscle alpha-actinin cDNA.
Sequence: View sequences (2)
Principal Investigator:
Carol Otey
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Dynamics of alpha-actinin in focal adhesions and stress fibers visualized with alpha-actinin-green fluorescent protein. Edlund et al (Cell Motil Cytoskeleton. 2001 Mar . 48(3):190-200. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11908" in your Materials and Methods section.