Addgene - pcDNA3-myc-FLNa WT Plasmid Data

Gene/insert name:
Filamin A
Alt name:
FLNa
Insert size:
8640
Species:
H. sapiens (human)
Entrez Gene:
FLNA (XX-FW83128A1.1, ABP-280, ABPX, CVD1, DKFZp434P031, FLJ43642, FLN, FLN-A, FLN1, FMD, MNS, NHBP, OPD, OPD1, OPD2, XLVD, XMVD)
Fusion protein or tag:
myc
Terminal:
N terminal on insert
Vector backbone:
pcDNA3
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
5446
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
John Blenis
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Ribosomal S6 kinase (RSK) regulates phosphorylation of filamin A on an important regulatory site. Woo et al (Mol Cell Biol. 2004 Apr . 24(7):3025-35. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8982" in your Materials and Methods section.