|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8982||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5446
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameFilamin A
SpeciesH. sapiens (human)
Insert Size (bp)8640
Entrez GeneFLNA (a.k.a. ABP-280, ABPX, CSBS, CVD1, FGS2, FLN, FLN-A, FLN1, FMD, MNS, NHBP, OPD, OPD1, OPD2, XLVD, XMVD)
/ Fusion Protein
- myc (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer pGEX-3 (Common Sequencing Primers)
Addgene sequencing results find amino acid substitution D2626H compared to reference sequence NP_001447.1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-myc-FLNa WT was a gift from John Blenis (Addgene plasmid # 8982 ; http://n2t.net/addgene:8982 ; RRID:Addgene_8982)
For your References section:Ribosomal S6 kinase (RSK) regulates phosphorylation of filamin A on an important regulatory site. Woo MS, Ohta Y, Rabinovitz I, Stossel TP, Blenis J. Mol Cell Biol. 2004 Apr . 24(7):3025-35. 10.1128/MCB.24.7.3025-3035.2004 PubMed 15024089