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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26768 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepShuttle-CMV
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Backbone manufacturerAgilent Technologies
- Backbone size w/o insert (bp) 7469
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Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTubulin
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SpeciesH. sapiens (human)
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Insert Size (bp)1600
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GenBank IDNM_006082
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Entrez GeneTUBA1B (a.k.a. K-ALPHA-1)
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Tag
/ Fusion Protein
- mCherry (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV-F, mCherry-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pShuttle mCherry-Tubulin was a gift from Torsten Wittmann (Addgene plasmid # 26768 ; http://n2t.net/addgene:26768 ; RRID:Addgene_26768) -
For your References section:
Analysis of microtubule dynamic instability using a plus-end growth marker. Matov A, Applegate K, Kumar P, Thoma C, Krek W, Danuser G, Wittmann T. Nat Methods. 2010 Sep . 7(9):761-8. 10.1038/nmeth.1493 PubMed 20729842