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pAdEasy mCherry-Tubulin
(Plasmid #26767)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pAdEasy-1
  • Backbone manufacturer
    Stratagene, ATCC
  • Backbone size w/o insert (bp) 33414
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Tubulin
  • Alt name
    TUBA1B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Entrez Gene
    TUBA1B (a.k.a. K-ALPHA-1)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

Resource Information

Depositor Comments

mCherry-Tubulin was cloned into pShuttle-CMV between KpnI and NotI then recombined into pAdEasy-1

AdEasy is a registered trademark of the Johns Hopkins University.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAdEasy mCherry-Tubulin was a gift from Torsten Wittmann (Addgene plasmid # 26767)
  • For your References section:

    Analysis of microtubule dynamic instability using a plus-end growth marker. Matov A, Applegate K, Kumar P, Thoma C, Krek W, Danuser G, Wittmann T. Nat Methods. 2010 Sep . 7(9):761-8. 10.1038/nmeth.1493 PubMed 20729842