Backbone manufacturerStratagene, ATCC
- Backbone size w/o insert (bp) 33414
Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
Copy numberLow Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
SpeciesH. sapiens (human)
Insert Size (bp)1600
Entrez GeneTUBA1B (a.k.a. K-ALPHA-1)
/ Fusion Protein
- mCherry (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F, mCherry-F (Common Sequencing Primers)
mCherry-Tubulin was cloned into pShuttle-CMV between KpnI and NotI then recombined into pAdEasy-1
AdEasy is a registered trademark of the Johns Hopkins University.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAdEasy mCherry-Tubulin was a gift from Torsten Wittmann (Addgene plasmid # 26767)
For your References section:Analysis of microtubule dynamic instability using a plus-end growth marker. Matov A, Applegate K, Kumar P, Thoma C, Krek W, Danuser G, Wittmann T. Nat Methods. 2010 Sep . 7(9):761-8. 10.1038/nmeth.1493 PubMed 20729842
Map generated by Addgene from plasmid data.