Addgene - pAdEasy mCherry-Tubulin Plasmid Data

Plasmid 26767: pAdEasy mCherry-Tubulin

Gene/insert name:
Tubulin
Alt name:
TUBA1B
Insert size:
1600
Species:
H. sapiens (human)
Entrez Gene:
TUBA1B (K-ALPHA-1)
Fusion protein or tag:
mCherry
Terminal:
N terminal on insert
Vector backbone:
pAdEasy-1
(Search Vector Database)
Backbone manufacturer:
Stratagene, ATCC
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
33414
5' sequencing primer:
CMV-F, mCherry-F List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (1)
Principal Investigator:
Torsten Wittmann
Terms and Licenses:
MTA
Clontech Limited Use Label License

Comments:

mCherry-Tubulin was cloned into pShuttle-CMV between KpnI and NotI then recombined into pAdEasy-1

AdEasy is a registered trademark of the Johns Hopkins University.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Analysis of microtubule dynamic instability using a plus-end growth marker. Matov et al (Nat Methods. 2010 Sep . 7(9):761-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26767" in your Materials and Methods section.