Addgene - pShuttle mCherry-Tubulin Plasmid Data

Plasmid 26768: pShuttle mCherry-Tubulin

Gene/insert name:
Tubulin
Insert size:
1600
Species:
H. sapiens (human)
GenBank ID:
NM_006082
Entrez Gene:
TUBA1B (K-ALPHA-1)
Fusion protein or tag:
mCherry
Terminal:
N terminal on insert
Vector backbone:
pShuttle-CMV
(Search Vector Database)
Backbone manufacturer:
Agilent Technologies
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
7469
Cloning site 5':
KpnI
Site destroyed during cloning:
Unknown
Cloning site 3':
NotI
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-F, mCherry-F List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Torsten Wittmann
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Analysis of microtubule dynamic instability using a plus-end growth marker. Matov et al (Nat Methods. 2010 Sep . 7(9):761-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26768" in your Materials and Methods section.