Addgene - pEnt L1L3-MCS Plasmid Data

Plasmid 26798: pEnt L1L3-MCS

Gene/insert name:
multiple cloning sequence
Alt name:
MCS
Insert size:
200
Vector backbone:
pEntr2B
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Gateway Entry vector
Backbone size w/o insert (bp):
2100
Cloning method:
Gateway Cloning
5' sequencing primer:
TCTACAAACTCTTCCTGTTAGT List of Sequencing Primers
3' sequencing primer:
AGAGATTTTGAGACACGGGC
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (4)
Map:
View map
Principal Investigator:
Edward Hsiao
Terms and Licenses:
MTA

Comments:

Empty entry vector carrying Gateway L1L3 sites, with MCS. This plasmid can act as an empty carrier when combined with a R3L2 plasmid to generate expression plasmids carrying just the R3L2 component (i.e., TetO-transgene expressing plasmid)

Plasmid was constructed as follows: The pEntr2B entry vector (Invitrogen) was digested with PstI and XhoI to remove the attL2 site.
Oligonucleotides containing SalI-XhoI-Bsu36I-NdeI-PacI-EcoRI-NotI flanking the attL3 sequence on the 5' side
and PstI on the 3' side were ligated in to create an intermediate plasmid carrying attL1 and attL3 sites. A LoxP sequence was introduced at the XhoI site.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice Hsiao et al (Stem Cell Res Ther. 2011 Mar 4;2(2):11. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26798" in your Materials and Methods section.