|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26804||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5404
Growth in Bacteria
Gene/Insert nameGuanidinoacetate N-methyltransferase
Alt nameGAMT promoter (full-length)
SpeciesH. sapiens (human)
Insert Size (bp)4500
Entrez GeneGAMT (a.k.a. PIG2, TP53I2)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
There are a few discrepancies between Addgene quality control sequence and NCBI reference sequences, however, these do not affect function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:-4200GAMT-P1-pGL4 was a gift from Sam Lee (Addgene plasmid # 26804 ; http://n2t.net/addgene:26804 ; RRID:Addgene_26804)
For your References section:GAMT, a p53-inducible modulator of apoptosis, is critical for the adaptive response to nutrient stress. Ide T, Brown-Endres L, Chu K, Ongusaha PP, Ohtsuka T, El-Deiry WS, Aaronson SA, Lee SW. Mol Cell. 2009 Nov 13. 36(3):379-92. 10.1016/j.molcel.2009.09.031 PubMed 19917247