|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26837||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5121
Modifications to backboneN/A
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
There are a few mismatches between author sequence and Addgene quality control sequence but they are outside of the URA3 coding sequence and do not alter function of this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pXP318 was a gift from Nancy DaSilva & Suzanne Sandmeyer (Addgene plasmid # 26837 ; http://n2t.net/addgene:26837 ; RRID:Addgene_26837)
For your References section:A vector set for systematic metabolic engineering in Saccharomyces cerevisiae. Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, Hatfield GW, Da Silva NA, Sandmeyer S. Yeast. 2010 Sep 10. ():. 10.1002/yea.1824 PubMed 20936606