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pXP418
(Plasmid #26843)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26843 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC18
  • Backbone manufacturer
    invitrogen
  • Backbone size (bp) 5976
  • Modifications to backbone
    N/A
  • Vector type
    Yeast Expression, Cre/Lox
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are a few mismatches between author sequence and Addgene quality control sequence but they are outside of the URA3 coding sequence and do not alter function of this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXP418 was a gift from Nancy DaSilva & Suzanne Sandmeyer (Addgene plasmid # 26843 ; http://n2t.net/addgene:26843 ; RRID:Addgene_26843)
  • For your References section:

    A vector set for systematic metabolic engineering in Saccharomyces cerevisiae. Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, Hatfield GW, Da Silva NA, Sandmeyer S. Yeast. 2010 Sep 10. ():. 10.1002/yea.1824 PubMed 20936606