Addgene - pXP522 Plasmid Data

Plasmid 26847: pXP522

Gene/insert name:
None
Vector backbone:
pUC18
(Search Vector Database)
Backbone manufacturer:
invitrogen
Vector type:
Yeast Expression, Cre/Lox
Backbone size (bp):
5150
Modifications to Backbone:
N/A
Cloning site 5':
SpeI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
N/A List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
LEU2
Sequence: View sequences (3)
Principal Investigator:
Nancy DaSilva
Principal Investigator:
Suzanne Sandmeyer
Terms and Licenses:
MTA

Comments:

There are a few mismatches between author sequence and Addgene quality control sequence but they are outside of the LEU2 coding sequence and do not alter function of this plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A vector set for systematic metabolic engineering in Saccharomyces cerevisiae. Fang et al (Yeast. 2010 Sep 10. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26847" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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