Ubp8/PET32a
(Plasmid
#26965)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26965 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePET32a
- Backbone size w/o insert (bp) 5900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameUbp8
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1416
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MutationTEV site is added t the 5' end and 4 alanines added between the TEV site and Ubp8 start site
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GenBank IDNM_001182730
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Entrez GeneUBP8 (a.k.a. YMR223W)
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Tags
/ Fusion Proteins
- His6 (N terminal on backbone)
- Trx (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ubp8/PET32a was a gift from Cynthia Wolberger (Addgene plasmid # 26965 ; http://n2t.net/addgene:26965 ; RRID:Addgene_26965) -
For your References section:
Structural insights into the assembly and function of the SAGA deubiquitinating module. Samara NL, Datta AB, Berndsen CE, Zhang X, Yao T, Cohen RE, Wolberger C. Science. 2010 May 21. 328(5981):1025-9. 10.1126/science.1190049 PubMed 20395473