pFastbac1-GST-hUSP7
(Plasmid #63573)

• Purpose: Expression of synthetic human USP7 tagged with GST
• Depositing Lab: Titia Sixma
• Publication: Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi: 10.1016/j.molcel.2011.06.034.

Backbone

• Vector backbone: pFastBac1
• Vector type: Insect Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: USP7
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 3309
• Mutation: Synthetic based on human protein sequence (please see depositor comment section below)
• Entrez Gene: USP7 (a.k.a. C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1)
• Promoter: Polyhedrin
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: Polyhedrin Forward (AAATGATAACCATCTCGC)
• 3′ sequencing primer: SV40 poly A Reverse

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the USP7 gene in this plasmid contains mutations S600P, F679L and V1020A when compared to the annotated gene/protein sequences within the Genbank/Uniprot databases. This plasmid should function as described in the associated publication.