PurposeExpression of synthetic human USP7 tagged with GST
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63573||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeInsect Expression
Growth in Bacteria
SpeciesH. sapiens (human), Synthetic
Insert Size (bp)3309
MutationSynthetic based on human protein sequence (please see depositor comment section below)
Entrez GeneUSP7 (a.k.a. HAFOUS, HAUSP, TEF1)
- Promoter Polyhedrin
/ Fusion Protein
- GST (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer Polyhedrin Forward (AAATGATAACCATCTCGC)
- 3′ sequencing primer SV40 poly A Reverse (Common Sequencing Primers)
Please note that the USP7 gene in this plasmid contains mutations S600P, F679L and V1020A when compared to the annotated gene/protein sequences within the Genbank/Uniprot databases. This plasmid should function as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFastbac1-GST-hUSP7 was a gift from Titia Sixma (Addgene plasmid # 63573 ; http://n2t.net/addgene:63573 ; RRID:Addgene_63573)
For your References section:Mechanism of USP7/HAUSP activation by its C-terminal ubiquitin-like domain and allosteric regulation by GMP-synthetase. Faesen AC, Dirac AM, Shanmugham A, Ovaa H, Perrakis A, Sixma TK. Mol Cell. 2011 Oct 7;44(1):147-59. doi: 10.1016/j.molcel.2011.06.034. 10.1016/j.molcel.2011.06.034 PubMed 21981925