pQHA-USP7 WT puroR
PurposeRetroviral vector that expresses wild type HA-tagged human USP7
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46753||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 10488
Modifications to backbonepQHA-puro was created by ligation into the MfeI- and AgeI-digested pQFlag-puro (pQFlag-puro was engineered by ligation in NotI- and BamHI-digested pQCXIP, giving the following MCS: NotI-Kozak-MfeI-Flag-AgeI-XhoI-BamHI).
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
SpeciesH. sapiens (human)
Entrez GeneUSP7 (a.k.a. HAFOUS, HAUSP, TEF1)
- Promoter CMV
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer pCDH-rev (Common Sequencing Primers)
Sequence discrepancies between the full and QC sequences have no functional consequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQHA-USP7 WT puroR was a gift from Goedele Maertens & Gordon Peters (Addgene plasmid # 46753 ; http://n2t.net/addgene:46753 ; RRID:Addgene_46753)
For your References section:Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation of the INK4a tumour suppressor. Maertens GN, El Messaoudi-Aubert S, Elderkin S, Hiom K, Peters G. EMBO J. 2010 Aug 4;29(15):2553-65. doi: 10.1038/emboj.2010.129. Epub 2010 Jul 2. 10.1038/emboj.2010.129 PubMed 20601937