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pQHA-USP7 CS puroR
(Plasmid #46754)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46754 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pQCXIP
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 10488
  • Modifications to backbone
    pQHA-puro was created by ligation into the MfeI- and AgeI-digested pQFlag-puro (pQFlag-puro was engineered by ligation in NotI- and BamHI-digested pQCXIP, giving the following MCS: NotI-Kozak-MfeI-Flag-AgeI-XhoI-BamHI).
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    USP7
  • Species
    H. sapiens (human)
  • Mutation
    C223S--catalytically inactive
  • Entrez Gene
    USP7 (a.k.a. HAUSP, TEF1)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer pCDH-Rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Sequence discrepancies between the full and QC sequences have no functional consequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQHA-USP7 CS puroR was a gift from Goedele Maertens & Gordon Peters (Addgene plasmid # 46754 ; http://n2t.net/addgene:46754 ; RRID:Addgene_46754)
  • For your References section:

    Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation of the INK4a tumour suppressor. Maertens GN, El Messaoudi-Aubert S, Elderkin S, Hiom K, Peters G. EMBO J. 2010 Aug 4;29(15):2553-65. doi: 10.1038/emboj.2010.129. Epub 2010 Jul 2. 10.1038/emboj.2010.129 PubMed 20601937