Addgene - pQCXIH-HA NEDD4L Plasmid Data

Plasmid 27028: pQCXIH-HA NEDD4L

Gene/insert name:
NEDD4L
Insert size:
3000
Species:
H. sapiens (human)
Entrez Gene:
NEDD4L (NEDD4-2, NEDD4.2, RSP5, hNEDD4-2)
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Vector backbone:
pQCXIH
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
7200
Cloning site 5':
NotI
Site destroyed during cloning:
Yes
Cloning site 3':
NotI
Site destroyed during cloning:
Yes
5' sequencing primer:
LNCX List of Sequencing Primers
3' sequencing primer:
IRES-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (1)
Principal Investigator:
Joan Massague
Terms and Licenses:
MTA

Comments:

Fragment from pCS2-HA NEDD4L, fill-in to make blunt-end insert.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta signaling. Gao et al (Mol Cell. 2009 Nov 13. 36(3):457-68. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 27028" in your Materials and Methods section.