pBiFC-VN155(I152L)
(Plasmid #27097)

• Depositing Lab: Chang-Deng Hu
• Publication: Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

Backbone

• Vector backbone: pMyc-CMV
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3791
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5 alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VN155(I152L)
• Alt name: Venus (1-154, I152L)
• Species: Aequorea victoria
• Insert Size (bp): 462
• Mutation: I152L
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 25 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To be published by Biotechniques in December. Please suggest the citation of our Biotechniques article. Also, this will be used as a replacement of pBiFC-VN173.