PurposeFor mammalian expression of the cytoplasmic domain of human ATF6with an HA tag.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27173||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7500
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1119
MutationContains only aa 1-373 (please see depositor comments below)
Entrez GeneATF6 (a.k.a. ACHM7, ATF6A)
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer Bglob-pA-R (Common Sequencing Primers)
This is the activated (nuclear) form of ATF6.
Please note that mutation M13I in ATF6 was found during Addgene's quality control. The plasmid should function as described in the associated publication above.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCGN-ATF6 (1-373) was a gift from Ron Prywes (Addgene plasmid # 27173 ; http://n2t.net/addgene:27173 ; RRID:Addgene_27173)
For your References section:Activation of ATF6 and an ATF6 DNA binding site by the endoplasmic reticulum stress response. Wang Y, Shen J, Arenzana N, Tirasophon W, Kaufman RJ, Prywes R. J Biol Chem. 2000 Sep 1. 275(35):27013-20. 10.1074/jbc.M003322200 PubMed 10856300