pCGN-ATF6 (1-373)
(Plasmid #27173)

• Purpose: For mammalian expression of the cytoplasmic domain of human ATF6with an HA tag.
• Depositing Lab: Ron Prywes
• Publication: Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-20.

Backbone

• Vector backbone: pCGN
• Backbone size w/o insert (bp): 7500
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ATF6
• Species: H. sapiens (human)
• Insert Size (bp): 1119
• Mutation: Contains only aa 1-373 (please see depositor comments below)
• Entrez Gene: ATF6 (a.k.a. ACHM7, ATF6A, ATP6alpha)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: Bglob-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the activated (nuclear) form of ATF6.
Please note that mutation M13I in ATF6 was found during Addgene's quality control. The plasmid should function as described in the associated publication above.