|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27233||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9066
Growth in Bacteria
/ Fusion Protein
- tandem dimer RFP
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Article Citing this Plasmid
Expresses tandem dimer RFP under the mouse α-CaMKII promoter (1.3kb fragment). Similar to plasmid # 27230, except for fluorescent tag.
Note that differences between Addgene's sequencing result and the sequence from the depositing scientist are due to use of theoretical maps. Please utilize Addgene's sequence when available.
EcoRI and BstBI sites are not present according to Addgene's sequencing results.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFCK(1.3)tdimer2W was a gift from Pavel Osten (Addgene plasmid # 27233 ; http://n2t.net/addgene:27233 ; RRID:Addgene_27233)
For your References section:Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo. Dittgen T, Nimmerjahn A, Komai S, Licznerski P, Waters J, Margrie TW, Helmchen F, Denk W, Brecht M, Osten P. Proc Natl Acad Sci U S A. 2004 Dec 28. 101(52):18206-11. 10.1073/pnas.0407976101 PubMed 15608064