|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27302||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4370
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameprom PDE4D3
SpeciesH. sapiens (human)
Insert Size (bp)1730
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NCOI (not destroyed)
- 5′ sequencing primer RV1 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL4.11 promPDE4D3 was a gift from David Fisher (Addgene plasmid # 27302 ; http://n2t.net/addgene:27302 ; RRID:Addgene_27302)
For your References section:Control of melanocyte differentiation by a MITF-PDE4D3 homeostatic circuit. Khaled M, Levy C, Fisher DE. Genes Dev. 2010 Oct 15. 24(20):2276-81. 10.1101/gad.1937710 PubMed 20952536