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NGFR
(Plasmid #27489)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    None
  • Species
    H. sapiens (human)
  • Tags / Fusion Proteins
    • ECMV (C terminal on insert)
    • IRES (C terminal on insert)
    • NGFR (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    truncated NGFR was from Warren Shlomchik
  • Terms and Licenses

Depositor Comments

MSCV ires NGFR (referred to as "NGFR") is identical to MigR1 except “ires GFP” of Mig R1 is replaced by the ECMV ires cloned in frame to the truncated human nerve growth factor receptor (tNGFR), which encodes the extracellular and transmembrane domains of hNGFR.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NGFR was a gift from Warren Pear (Addgene plasmid # 27489)
  • For your References section:

    Notch1 regulates maturation of CD4+ and CD8+ thymocytes by modulating TCR signal strength. Izon DJ, Punt JA, Xu L, Karnell FG, Allman D, Myung PS, Boerth NJ, Pui JC, Koretzky GA, Pear WS. Immunity. 2001 Mar . 14(3):253-64. 10.1016/S1074-7613(01)00107-8 PubMed 11290335