Plasmid 27489: NGFR

• Gene/insert name: None
• Species: H. sapiens (human)
• Fusion protein or tag: ECMV
• Terminal: C terminal on insert
• Fusion protein or tag: IRES
• Terminal: C terminal on insert
• Fusion protein or tag: NGFR
• Terminal: C terminal on insert
• Vector backbone: MSCV2.2 (MIG)
  (Search Vector Database)
• Vector type: Mammalian Expression, Retroviral
• 5' sequencing primer: pLXSN 5'
• Bacterial resistance(s) Ampicillin
• Growth strain(s) DH5alpha
• Growth temperature (℃): 37
• High or low copy: High Copy
• Person or lab that originally
  cloned the gene/insert: truncated NGFR was from Warren Shlomchik
• Sequence: View sequences (2)
• Map: View map
• Principal Investigator: Warren Pear
• Terms and Licenses: MTA

Comments: 

MSCV ires NGFR (referred to as "NGFR") is identical to MigR1 except “ires GFP” of Mig R1 is replaced by the ECMV ires cloned in frame to the truncated human nerve growth factor receptor (tNGFR), which encodes the extracellular and transmembrane domains of hNGFR.

Article: Notch1 regulates maturation of CD4+ and CD8+ thymocytes by modulating TCR signal strength. Izon et al (Immunity. 2001 Mar . 14(3):253-64. PubMed)