|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27493||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4200
Vector typeMammalian Expression
Growth in Bacteria
Growth instructionsDH5alpha 37 degree in standard LB buffer
Copy numberHigh Copy
Gene/Insert nameNuclear localization signal-Cre
Insert Size (bp)1970
/ Fusion Protein
- nuclear localization signal (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site BbsI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
Although the kanamycin resistance marker is present in the full plasmid sequence, the depositing lab has never used Kan or Kan/Amp selection for this plasmid. In Addgene's experience, the kanamycin resistance is not functional. According to the Luo lab, the function of this plasmid is not affected.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBT140 was a gift from Liqun Luo (Addgene plasmid # 27493 ; http://n2t.net/addgene:27493 ; RRID:Addgene_27493)
For your References section:Cortical representations of olfactory input by trans-synaptic tracing. Miyamichi K, Amat F, Moussavi F, Wang C, Wickersham I, Wall NR, Taniguchi H, Tasic B, Huang ZJ, He Z, Callaway EM, Horowitz MA, Luo L. Nature. 2010 Dec 22. ():. 10.1038/nature09714 PubMed 21179085