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YIPlac204TC-NLS-DsRed-Express2
(Plasmid #25443)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25443 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    YIPlac204
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NLS-DsRed-Express2
  • Alt name
    DsRed-Express2
  • Species
    Discosoma sp.
  • Insert Size (bp)
    714
  • GenBank ID
    FJ226077
  • Tag / Fusion Protein
    • NLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer ACAGGTGGTTTGTTACGCATGCTAATGC
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YIPlac204TC-NLS-DsRed-Express2 was a gift from Benjamin Glick (Addgene plasmid # 25443 ; http://n2t.net/addgene:25443 ; RRID:Addgene_25443)
  • For your References section:

    A noncytotoxic DsRed variant for whole-cell labeling. Strack RL, Strongin DE, Bhattacharyya D, Tao W, Berman A, Broxmeyer HE, Keenan RJ, Glick BS. Nat Methods. 2008 Nov . 5(11):955-7. 10.1038/nmeth.1264 PubMed 18953349