|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27511||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2500
Vector typeWorm Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesC. elegans (nematode)
Insert Size (bp)4000
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPD162.21 was a gift from Andrew Fire (Addgene plasmid # 27511 ; http://n2t.net/addgene:27511 ; RRID:Addgene_27511)
For your References section:Cell autonomous specification of temporal identity by Caenorhabditis elegans microRNA lin-4. Zhang H, Fire AZ. Dev Biol. 2010 Aug 15. 344(2):603-10. 10.1016/j.ydbio.2010.05.018 PubMed 20493184