Plasmid 26347: pBCN27-Pmyo-2::GFP::unc-54_3'UTR

• Gene/insert name: Pmyo-2::GFP::unc-54_3'UTR
• Species: C. elegans (nematode)
• Vector backbone: pBCN27-R4R3
  (Search Vector Database)
• Vector type: Worm Expression
• Backbone size w/o insert (bp): 10813
• Cloning site 5': AttR4
• Site destroyed during cloning: Yes
• Cloning site 3': AttR3
• Site destroyed during cloning: Yes
• 5' sequencing primer: n/a
• Bacterial resistance(s) Ampicillin
• Growth strain(s) DH5alpha
• Growth temperature (℃): 37
• High or low copy: Unknown
• Selectable markers: Puromycin
• Selectable markers, other: unc-119
• Person or lab that originally
  cloned the gene/insert: Puromycin resistance gene from pBabePuro (Addgene). rpl-28 promoter and let-858 3'UTR sequences from pPD129.57 vector (Addgene).
• Sequence: View sequences (4)
• Principal Investigator: Ben Lehner
• Terms and Licenses: MTA

Comments: 

The full sequence provided is theoretical sequence based on a three way gateway reaction. The order of the elements has been confirmed by PCR, but not fully sequence verified. Expression pattern in worms is as expected.

Use this plasmid as a positive control for testing puromycin selection after single copy insertion by MosSCI at the ttTi5605 locus.

Article: Rapid selection of transgenic C. elegans using antibiotic resistance. Semple et al (Nat Methods. 2010 Aug 22. ():. PubMed)