pBCN27-Pmyo-2::GFP::unc-54_3'UTR
(Plasmid #26347)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBCN27-R4R3
  • Backbone size w/o insert (bp) 10813
  • Vector type
    Worm Expression
  • Selectable markers
    Puromycin ; unc-119

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    Pmyo-2::GFP::unc-54_3'UTR
  • Species
    C. elegans (nematode)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AttR4 (destroyed during cloning)
  • 3′ cloning site AttR3 (destroyed during cloning)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The full sequence provided is theoretical sequence based on a three way gateway reaction. The order of the elements has been confirmed by PCR, but not fully sequence verified. Expression pattern in worms is as expected.

Use this plasmid as a positive control for testing puromycin selection after single copy insertion by MosSCI at the ttTi5605 locus.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBCN27-Pmyo-2::GFP::unc-54_3'UTR was a gift from Ben Lehner (Addgene plasmid # 26347)
  • For your References section:

    Rapid selection of transgenic C. elegans using antibiotic resistance. Semple JI, Garcia-Verdugo R, Lehner B. Nat Methods. 2010 Aug 22. ():. 10.1038/nmeth.1495 PubMed 20729840