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Plasmid 26347: pBCN27-Pmyo-2::GFP::unc-54_3'UTR
  • Pmyo-2::GFP::unc-54_3'UTR

  • C. elegans (nematode)

  • pBCN27-R4R3
    (Search Vector Database)

  • Worm Expression

  • 10813

  • AttR4

  • Yes

  • AttR3

  • Yes

  • n/a List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • Unknown

  • Puromycin ; unc-119

  • Puromycin resistance gene from pBabePuro (Addgene). rpl-28 promoter and let-858 3'UTR sequences from pPD129.57 vector (Addgene).

  • View sequences (4)
  • Ben Lehner

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

The full sequence provided is theoretical sequence based on a three way gateway reaction. The order of the elements has been confirmed by PCR, but not fully sequence verified. Expression pattern in worms is as expected.

Use this plasmid as a positive control for testing puromycin selection after single copy insertion by MosSCI at the ttTi5605 locus.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Rapid selection of transgenic C. elegans using antibiotic resistance. Semple et al (Nat Methods. 2010 Aug 22. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26347" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only