Addgene - pQCXIN- myc ULK1 wt Plasmid Data

Plasmid 27626: pQCXIN- myc ULK1 wt

Gene/insert name:
mouse myc ULK1
Insert size:
3192
Species:
M. musculus (mouse)
Fusion protein or tag:
myc
Terminal:
N terminal on insert
Mutation:
Initial M from ULK1 removed. Silent mutation at Arginine 5. Does not affect amino acid sequence.
Vector backbone:
pQCXIN Neo dest
(Search Vector Database)
Backbone manufacturer:
Addgene plasmid 17399
Vector type:
Retroviral
Backbone size w/o insert (bp):
9135
Cloning method:
Gateway Cloning
Cloning site 5':
none/attR1
Site destroyed during cloning:
Unknown
Cloning site 3':
none/attR2
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
30
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (1)
Principal Investigator:
Reuben Shaw
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Phosphorylation of ULK1 (hATG1) by AMP-Activated Protein Kinase Connects Energy Sensing to Mitophagy. Egan et al (Science. 2010 Dec 23. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 27626" in your Materials and Methods section.