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pEBG‐AMPK
α1(1‐312)
(Plasmid #27632)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27632 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AMPK
α1(1‐312)
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2400
  • Mutation
    Constitutively
 active
 by 
truncation 
after 
amino 
acid 
312.
  • Entrez Gene
    Prkaa1
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer EF-1a Forward
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid was first described in the following article: Crute et al. Functional domains of the alpha1 catalytic subunit of the AMP-activated protein kinase. J Biol Chem (1998) vol. 273 (52) pp. 35347-54.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBG‐AMPK
α1(1‐312) was a gift from Reuben Shaw (Addgene plasmid # 27632 ; http://n2t.net/addgene:27632 ; RRID:Addgene_27632)
  • For your References section:

    Phosphorylation of ULK1 (hATG1) by AMP-Activated Protein Kinase Connects Energy Sensing to Mitophagy. Egan DF, Shackelford DB, Mihaylova MM, Gelino SR, Kohnz RA, Mair W, Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ. Science. 2010 Dec 23. ():. 10.1126/science.1196371 PubMed 21205641