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ampkar
(Plasmid #35097)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35097 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ampkar
  • Species
    artificial
  • Insert Size (bp)
    2300
  • Mutation
    na
  • GenBank ID
    na
  • Promoter CMV
  • Tags / Fusion Proteins
    • ECFP (N terminal on insert)
    • Venus (C terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ampkar was a gift from Lewis Cantley (Addgene plasmid # 35097 ; http://n2t.net/addgene:35097 ; RRID:Addgene_35097)
  • For your References section:

    A fluorescent reporter of AMPK activity and cellular energy stress. Tsou P, Zheng B, Hsu CH, Sasaki AT, Cantley LC. Cell Metab. 2011 Apr 6;13(4):476-86. 10.1016/j.cmet.2011.03.006 PubMed 21459332