Addgene - ampkar Plasmid Data

Plasmid 35097: ampkar

Gene/insert name:
ampkar
Insert size:
2300
Species:
artificial
GenBank ID:
na
Fusion protein or tag:
ECFP
Terminal:
N terminal on insert
Fusion protein or tag:
Venus
Terminal:
C terminal on insert
Mutation:
na
Vector backbone:
pcDNA3
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5400
Promoter:
CMV
Cloning method:
Ligation Independent Cloning
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (4)
Map:
View map
Principal Investigator:
Lewis Cantley
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A fluorescent reporter of AMPK activity and cellular energy stress. Tsou et al (Cell Metab. 2011 Apr 6;13(4):476-86. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35097" in your Materials and Methods section.