|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27796||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepECFP C1
- Backbone size w/o insert (bp) 3984
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemCerulean C1
Insert Size (bp)720
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe 1 (not destroyed)
- 3′ cloning site Bam HI (not destroyed)
- 5′ sequencing primer CCAAAATCAACGGGACTTTCC
- 3′ sequencing primer CAGGTTCAGGGGGAGGTGTGG (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
This construct was created by mutagenesis of pECFP-C1. When compared with ECFP, Cerulean contains the following mutations: S72A, Y145A and H148D. This construct also contains A206K mutation to create a monomeric form of the fluorescent protein.
See Addgene's sequencing results for detailed mCerulean and MCS. This plasmid is designed to clone a gene of interest downstream and in-frame to create a N-terminal mCerulean fusion protein.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mCerulean C1 was a gift from Steven Vogel (Addgene plasmid # 27796 ; http://n2t.net/addgene:27796 ; RRID:Addgene_27796)
For your References section:Cerulean, Venus, and VenusY67C FRET reference standards. Koushik SV, Chen H, Thaler C, Puhl HL, Vogel SS. Biophys J. 2006 Dec 15. 91(12):L99-L101. 10.1529/biophysj.106.096206 PubMed 17040988