Addgene - pLenti-EF1a-Backbone(NN) Plasmid Data

Plasmid 27961: pLenti-EF1a-Backbone(NN)

Gene/insert name:
TAL-Backbone(NN)
Alt name:
Backbone(NN)
Insert size:
2349
Species:
Synthetic
Vector backbone:
pLenti
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
9204
Cloning site 5':
BsiWI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
TTTTGAGTTTGGATCTTGGT List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Feng Zhang
Terms and Licenses:
MTA

Comments:

Please visit www.taleffectors.com for detailed protocols and plasmid maps.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al (Nat Biotechnol. 2011 Jan 19. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 27961" in your Materials and Methods section.