pAAV-minCMV-mCherry
(Plasmid #27970)

Depositing Lab

Feng Zhang
Publication

Zhang et al Nat Biotechnol. 2011 Jan 19. ():.
Price

$65 (USD)

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Available to Academic and Nonprofits Only

Backbone

Vector backbone
pAAV
(Search Vector Database)
Backbone size w/o insert (bp) 4154
Vector type
Mammalian Expression

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Sequence Information

Depositor Sequences

Full (1)
Addgene Sequences

Partial (3)

Gene/Insert

Gene/Insert name
mCherry
Species
Synthetic
Insert Size (bp)
711

Cloning Information

Cloning method Restriction Enzyme
5′ cloning site MluI (not destroyed)
3′ cloning site EcoRI (not destroyed)
5′ sequencing primer ccgagggcttcaagtgggagcgc
3′ sequencing primer cagcttcaccttgtagatgaactcgc

(Common Sequencing Primers)

Resource Information

Addgene Notes
- Addgene diagnostic digest on replacement
Terms and Licenses

Depositor Comments

Please visit www.taleffectors.com for detailed protocols and plasmid maps.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pAAV-minCMV-mCherry was a gift from Feng Zhang (Addgene plasmid # 27970)
For your References section:

Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P. Nat Biotechnol. 2011 Jan 19. ():. 10.1038/nbt.1775 PubMed 21248753

Map generated by Addgene from full sequence supplied by depositor.

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pAAV-minCMV-mCherry (Plasmid #27970)