pAAV-minCMV-mCherry
(Plasmid #27970)

• Depositing Lab: Feng Zhang
• Publication: Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4154
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry
• Species: Synthetic
• Insert Size (bp): 711

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: ccgagggcttcaagtgggagcgc
• 3′ sequencing primer: cagcttcaccttgtagatgaactcgc

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest on replacement
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the left ITR has an 11 bp deletion that removes the C' arm.