Addgene - pAAV-minCMV-mCherry Plasmid Data

Plasmid 27970: pAAV-minCMV-mCherry

Gene/insert name:
mCherry
Insert size:
711
Species:
Synthetic
Vector backbone:
pAAV
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4154
Cloning site 5':
MluI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
ccgagggcttcaagtgggagcgc List of Sequencing Primers
3' sequencing primer:
cagcttcaccttgtagatgaactcgc
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (4)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Feng Zhang
Terms and Licenses:
UBMTA
Clontech Limited Use Label License
Ancillary Agreement for Plasmids Containing FP Materials

Comments:

Please visit www.taleffectors.com for detailed protocols and plasmid maps.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al (Nat Biotechnol. 2011 Jan 19. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 27970" in your Materials and Methods section.