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(Plasmid #27970)

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Available to Academic and Nonprofits Only


  • Vector backbone
  • Backbone size w/o insert (bp) 4154
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Sequence Information


  • Gene/Insert name
  • Species
  • Insert Size (bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer ccgagggcttcaagtgggagcgc
  • 3′ sequencing primer cagcttcaccttgtagatgaactcgc
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please visit for detailed protocols and plasmid maps.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-minCMV-mCherry was a gift from Feng Zhang (Addgene plasmid # 27970)
  • For your References section:

    Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P. Nat Biotechnol. 2011 Jan 19. ():. 10.1038/nbt.1775 PubMed 21248753