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pEF1a-Ronin-FLAG-IRES-Neo
(Plasmid #28020)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28020 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEF1a-Luc-IRES-Neo
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ronin
  • Alt name
    Thap11
  • Alt name
    pTZ1133
  • Species
    H. sapiens (human)
  • Entrez Gene
    THAP11 (a.k.a. HRIHFB2206, CTG-B43a, CTG-B45d, RONIN)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    To obtain the pEF1a-Ronin-Flag-Ires-Neo vector, we cloned the PCR product into pEF1-Luc-IRES-Neo (the kind gift of David Spencer, Baylor College of Medicine) using BglII and XbaI, replacing the luciferase gene with Ronin
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Even though the XbaI site is still present, XbaI does not cut this plasmid anymore possibly due to methylation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1a-Ronin-FLAG-IRES-Neo was a gift from Thomas Zwaka (Addgene plasmid # 28020 ; http://n2t.net/addgene:28020 ; RRID:Addgene_28020)
  • For your References section:

    Ronin/Hcf-1 binds to a hyperconserved enhancer element and regulates genes involved in the growth of embryonic stem cells. Dejosez M, Levine SS, Frampton GM, Whyte WA, Stratton SA, Barton MC, Gunaratne PH, Young RA, Zwaka TP. Genes Dev. 2010 Jul 15. 24(14):1479-84. 10.1101/gad.1935210 PubMed 20581084