|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||28021||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5633
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationDHSA mutation (Y246A) of the previously defined HCF-1-binding motif. By yeast two-hybrid assay, this change completely abolished the ability of Ronin to interact with Hcf-1
Entrez GeneTHAP11 (a.k.a. HRIHFB2206, CTG-B43a, CTG-B45d, RONIN)
/ Fusion Protein
- FLAG (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Even though the XbaI site is still present, XbaI does not cut this plasmid anymore, possibly due to methylation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEF1a-RoninDHSA-FLAG-IRES-Neo was a gift from Thomas Zwaka (Addgene plasmid # 28021 ; http://n2t.net/addgene:28021 ; RRID:Addgene_28021)
For your References section:Ronin/Hcf-1 binds to a hyperconserved enhancer element and regulates genes involved in the growth of embryonic stem cells. Dejosez M, Levine SS, Frampton GM, Whyte WA, Stratton SA, Barton MC, Gunaratne PH, Young RA, Zwaka TP. Genes Dev. 2010 Jul 15. 24(14):1479-84. 10.1101/gad.1935210 PubMed 20581084