Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #28021)


Item Catalog # Description Quantity Price (USD)
Plasmid 28021 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5633
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Mutation
    DHSA mutation (Y246A) of the previously defined HCF-1-binding motif. By yeast two-hybrid assay, this change completely abolished the ability of Ronin to interact with Hcf-1
  • Entrez Gene
    THAP11 (a.k.a. HRIHFB2206, CTG-B43a, CTG-B45d, RONIN)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    To obtain the pEF1a-Ronin-Flag-Ires-Neo vector, we cloned the PCR product into pEF1-Luc-IRES-Neo (the kind gift of David Spencer, Baylor College of Medicine) using BglII and XbaI, replacing the luciferase gene with Ronin. The DHSA mutation (Y246A) was introduced by site-directed mutagenesis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Even though the XbaI site is still present, XbaI does not cut this plasmid anymore, possibly due to methylation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1a-RoninDHSA-FLAG-IRES-Neo was a gift from Thomas Zwaka (Addgene plasmid # 28021 ; ; RRID:Addgene_28021)
  • For your References section:

    Ronin/Hcf-1 binds to a hyperconserved enhancer element and regulates genes involved in the growth of embryonic stem cells. Dejosez M, Levine SS, Frampton GM, Whyte WA, Stratton SA, Barton MC, Gunaratne PH, Young RA, Zwaka TP. Genes Dev. 2010 Jul 15. 24(14):1479-84. 10.1101/gad.1935210 PubMed 20581084